Rabbit acetylcholine,ACH ELISA Kit->biometric sensors

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Rabbit acetylcholine,ACH ELISA Kit

Category :

Biotech->Others->biometric sensors

Product Specification

Model No:	E0912Rb

Country	CN
Brand	USCNLIFE
Minimum Order	1Kit
Payment Term	T/T

Product Detail

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Intended use
This immunoassay kit allows for the specific measurement of rabbit Acetylcholine concentrations in cell culture supernates, serum, and plasma.

Introduction
Acetylcholine (Ach) is the endogenous agonist of nAChRs and is synthesized and stored in ECs and
blood cells, ACh was first identified by Henry Hallett Dale for its actions on heart tissue. It is an ester of choline and acetic acid that is the transmitter substance at many neural, or nerve, synapses and at the motor end plate of vertebrate muscles. When a nerve impulse arrives at the nerve ending, ACh, which is stored there in vesicles, is released and combines with a receptor molecule in the postsynaptic membrane or the end-plate membrane of a muscle fibre. This bonding changes the permeability of the membrane, and a change in the nature of a generator potential results. The effects
of successive nerve impulses accumulate if they arrive at a sufficiently high frequency. The ACh is destroyed by an enzyme, acetylcholinesterase, and thus is effective only briefly. Inhibitors of the enzyme, however, prolong the lifetime of ACh itself. ACh affects a number of body systems including the cardiovascular system by acting as a vasodilator, by decreasing cardiac rate, and by decreasing cardiac contraction; the gastrointestinal system by such activities as increasing peristalsis in the
stomach and by increasing the amplitude of digestive contractions; and the urinary tract by such actions as decreasing the capacity of the bladder and increasing the voluntary voiding pressure. It also affects the respiratory system and stimulates secretion by all glands that receive parasympathetic nerve impulses

Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal
antibody specific for Acetylcholine has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Acetylcholine present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Acetylcholine is added to the wells. Following a
wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Acetylcholine bound in the initial step. The color development is stopped and the intensity of the color is measured.

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